TransGLYCIT
• Human IgG1, IgG2 and IgG4
• 3-6 hour workflow
• Glycoform Azide is included
• Transglycosylation occurs at the Fc N-glycan sites
TransGLYCIT는 효율적이고 특이적 human IgG Glycan remodeling 및 Conjugation을 가능하게 하는 플랫폼입니다. TransGLYCIT를 사용하면 동일한 glycoform 형성 또는 azide activation을 통해 Antibody가 빠르고 강하게 생성됩니다. Glycan에 FucosEXO™ 16 enzyme (옵션)을 사용하면, 선택한 Fc Glycoform (G0, G1, G2 또는 G2S2)을 운반하는 afucosylated antibody가 형성되어 core fucose가 있거나 없는 antibody 비교를 할 수 있습니다. Azide activation work flow를 사용하면 항체는 원하는 부분에 labeling과 conjugation을 할 수 있습니다.
The Azide Activation Transglycosylation Workflow
Deglycosylation
The Fc N-glycans are trimmed to the core GlcNAc using the IgG-specific Immobilized GlycINATOR® (EndoS2) enzyme that hydrolyses all Fc glycoforms, including high-mannose, hybrid, complex and bisecting glycans.
Transglycosylation
The engineered glycosynthase TransINATOR™ catalyzes the transglycosylation reaction between the oxazoline glycoform azide and the core GlcNAc. The degree of fucosylation will be the same as on the original IgG.
The transglycosylation of IgG is followed by an affinity purification step.
Content and Storage
TransGLYCIT Azide Activation is shipped cold, and the content should be stored at different temperatures upon arrival. TransGLYCIT is provided in kits containing sufficient material to transglycosylate 100 µg human IgG. TransGLYCIT is for R&D use only.
The TransGLYCIT Azide Activation kits contain:
- Immobilized GlycINATOR
- TransINATOR enzyme
- Oxazoline glycoform Azide
- CaptureSelect™ spin column for purification
TransGLYCIT™
| Part number | Item name | Description |
| T1-G0F-010 | TransGLYCIT G0, 1 mg | Generates 1 mg human IgG with the G0 glycoform |
| T1-G1F-010 | TransGLYCIT G1, 1 mg | Generates 1 mg human IgG with the G1 glycoform |
| T1-G2F-010 | TransGLYCIT G2, 1 mg | Generates 1 mg human IgG with the G2 glycoform |
| T1-S2F-010 | TransGLYCIT G2S2, 1 mg | Generates 1 mg human IgG with the G2S2 glycoform |
TransGLYCIT™ Azide Activation
| Part number | Item name | Description |
| T1-AZ1-001 | TransGLYCIT Azide Activation, 100 μg | Activates 100 μg human IgG1 or IgG4 |
| T1-AZ2-001 | Activates 100 μg human IgG2 | TransGLYCIT Azide Activation hIgG2, 100 μg |
TransGLYCIT™ Afucosylated
| Part number | Item name | Description |
| T1-G0A-010 | TransGLYCIT G0 Afucosylated, 1 mg | Generates 1 mg afucosylated human IgG with the G0 glycoform |
| T1-G1A-010 | TransGLYCIT G1 Afucosylated, 1 mg | Generates 1 mg afucosylated human IgG with the G1 glycoform |
| T1-G2A-010 | TransGLYCIT G2 Afucosylated, 1 mg | Generates 1 mg afucosylated human IgG with the G2 glycoform |
| T1-S2A-010 | TransGLYCIT G2S2 Afucosylated, 1 mg | Generates 1 mg afucosylated human IgG with the G2S2 glycoform |