Epigenetic 서비스
Eurofins Discovery
| Eurofins Discover (Eurofins Discovery) 공식 대리점
INTRODUCTION
Eurofins Discovery에서는 Epigenetics에서 주요한 역할을 하고 있다고 알려진 타겟들에 대한 분석 Assay를 제공합니다.
KINOMEscan Technology를 기반으로 하여 Bromo domain과의 binding 능력을 측정하는 BROMOscan 서비스와 InCELL Hunter™ technology를 기반으로 하여 세포 내 환경에서 Compound의 Permeability와 Binding을 직접적으로 측정할 수 있는 EPIscan Cell-based Assay, Enzymatic Assay가 있습니다.
Biswas S, Rao CM. Epigenetic tools (The Writers, The Readers and The Erasers) and their implications in cancer therapy. Eur J Pharmacol. 2018;837:8-24. doi:10.1016/j.ejphar.2018.08.021
- Bromo domain을 타겟으로 한 40+개의 Quantitative Binding Assays
- Writer 타겟: SMMT, Kinase, DNMT, HMT, HAT, etc.
- Eraser 타겟: Demethylase, HDAC / sitruin, etc.
서비스 종류 및 상세
BROMOscan 서비스
| 서비스 유형 | bromoMAX | bromoELECT | bromoKdMAX | bromoKdELECT |
| Assay 수 | 32 | 1-32 | 40 | 1-40 |
| Class | Reader | |||
| Assay | Binding Assay | |||
| 실험농도 | 선택 가능 | |||
| 농도 개수 | 1 | 11개 농도(Top concentration에서 dilution) | ||
| 반복실험횟수 | Duplicate | |||
| Sample Volume | 실험할 농도의 1000X stock으로 50 uL | |||
| TAT | 20 Business Days | |||
| 서비스 장소 | San Diego, CA USA | |||
EPIscan Cell-Based Assay 서비스
EPIscan Screening & Profiling 서비스는 Cell-based Bromodomain & Histone Methyltransferase (HMT) Assay로, InCELL Hunter™ technology를 통해 세포 내 환경에서 Compound의 Permeability와 Binding을 직접적으로 측정할 수 있습니다.
- Primary Screening, Lead Optimization, SAR 등의 다양한 Application에 이용 가능
- Cellular Potency에 따른 Compound의 Rank Ordering
- 재현 가능한 고품질의 데이터 생성을 통한 연구 시간 단축
| 서비스 유형 | EPIscan |
| Assay Type | Cell-based Functional Assay |
| 실험농도 | 선택 가능 |
| 농도 개수 | 선택 가능 |
| 반복실험횟수 | Duplicate |
| Sample Volume | 실험할 농도의 1000X stock으로 40 uL |
| TAT | 15 Business Days |
Enzymatic Assay
| Class | Assay Targets | Service | Product | |
| Item no. | Cell line | Kit | ||
| Writer | EHMT1 Human Histone Methyltransferase (HMT) Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2404AG | ||
| G9A Human Histone Methyltransferase (HMT) Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2403AG | O | O | |
| PRMT3 Human Protein Arginine N-Methyltransferase Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2393AG | O | ||
| Reader | BAZ2A Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2392AG | O | |
| BRD2 (1) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2397AG | O | O | |
| BRD2 (1,2) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2394AG | O | ||
| BRD3 (1) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2399AG | O | ||
| BRD3 (1,2) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2398AG | O | O | |
| BRD4 (1) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2400AG | O | O | |
| BRDT (1) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2402AG | O | ||
| BRDT (1,2) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2401AG | O | O | |
| SMARCA4 Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2396AG | O | O | |
| TAF1L (2) Human Bromodomain-Containing Cell Based Agonist Binding LeadHunter Assay – US | 85-0001P-2395AG | O | ||
| Kinase | BLK Human TK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2441AG | O | O |
| BTK Human TK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2436AG | O | O | |
| CK1delta (CSNK1D) Human CK1 Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2433AG | O | O | |
| CLK1 Human CMGC Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2434AG | O | O | |
| CLK2 Human CMGC Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2435AG | O | O | |
| FGR Human TK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2442AG | O | ||
| HCK Human TK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2443AG | O | O | |
| MEK1 Human STE Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2444AG | O | O | |
| PI3Kdelta (PIK3CD) Human PtdIns(4,5)P 3-Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2439AG | O | ||
| Pim1 Human CAMK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2431AG | O | O | |
| PYK2 Human TK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2438AG | O | O | |
| RIPK1 Human TKL Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2430AG | O | ||
| RIPK2 Human TKL Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2440AG | O | O | |
| RIPK3 Human TKL Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2429AG | O | ||
| SIK Human CAMK Kinase Cell Based Agonist Binding LeadHunter Assay – US | 86-0022P-2432AG | O | O | |
| Apoptosis | B2CL1-BAX Human Pathways Cell Based Agonist Protein Binding LeadHunter Assay – US | 86-0016P-2648AG | O | O |
| B2CL1-BIM Human Pathways Cell Based Agonist Protein Binding LeadHunter Assay – US | 86-0016P-2649AG | O | ||
| BCL2-BAX Human Pathways Cell Based Agonist Protein Binding LeadHunter Assay – US | 86-0016P-2646AG | O | ||
| BCL2-BIM Human Pathways Cell Based Agonist Protein Binding LeadHunter Assay – US | 86-0016P-2647AG | O | ||
| MCL1-BIM Human Pathways Cell Based Agonist Protein Binding LeadHunter Assay – US | 86-0016P-2645AG | O | ||
Related Service
Related Product
InCELL Hunter™ Cell Line
Native 세포 환경에서 화합물 투과성 및 결합을 직접 측정할 수 있도록 하는 Cell Line으로, Intracellular Binding에 대한 데이터를 얻을 수 있습니다.
| Cell Background | Target | Cat. No. |
| A549 | EP300 Bromodomain | 96-0073C15 |
| G9a Methyltransferase | 96-0003C15 | |
| GLP Methyltransferase | 96-0045C15 | |
| PRMT3 Methyltransferase | 96-0072C15 | |
| HEK 293 | BAZ2A Bromodomain | 96-0074C1 |
| BRD2(1,2) Bromodomain | 96-0049C1 | |
| BRD2(1) Bromodomain | 96-0004C1 | |
| BRD3(1,2) Bromodomain | 96-0038C1 | |
| BRD3(1) Bromodomain | 96-0036C1 | |
| BRD4(1) Bromodomain | 96-0005C1 | |
| BRDT(1,2) Bromodomain | 96-0046C1 | |
| BRDT(1) Bromodomain | 96-0006C1 | |
| SMARCA4 Bromodomain | 96-0060C1 | |
| TAF1L(2) Bromodomain | 96-0058C1 |
SPRINTer™ Cell Line
Protein Turnover Detection을 위한 세포 기반 분석이 가능한 Cell Line으로, 간편한 실험 프로토콜과 빠른 실험 결과 도출이 특징입니다.
| Target | Description | Cat. No. |
| BRD4 | SPRINTer™ HCT-116 BRD4 Protein Turnover Biosensor Cell Line | 91-1004C047 |
| BRD4 | SPRINTer™ K562 BRD4 Protein Turnover Biosensor Cell Line | 91-1002C042 |
서비스 절차
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