IonChannelProfiler™ Services
Manual Patch Clamp와 Automated Patch Clamp platform을 통해 후보 약물의 Ion channel target에 대한 selectivity와 심장부작용을 평가할 수 있는 서비스
| Eurofins DiscoverX(Eurofins Discovery) 공식 대리점
INTRODUCTION
- Ion channel은 부정맥, 고혈압, 뇌졸증, 간질, 우울증, COPD, 당뇨병 등 다양한 질병 치료제 개발을 위한 중요한 표적이자 안전성 평가를 위한 주요 항목입니다.
- Eurofins Discovery는 60개 이상의 PrecisION® voltage-gated, ligand-gated ion channel cell lines을 사용하여 binding assay 및 functional assay를 지원합니다.
- 전문가들로 구성된 Eurofins Discovery Ion Channel Center를 통해 적절한 assay mode (binding 또는 functional assay) 및 platform (manual 또는 automated patch clamp) 선택을 위한 가이드를 제공받으실 수 있습니다.
서비스 종류
| Ion Channel Family/Subtype |
Service | Product | ||||||
| Functional Assay | Binding Assays | |||||||
| Manual Patch Clamp |
QPatch Automated Patch Clamp | Qube Automated Patch Clamp |
IonFlux Automated Patch Clamp |
FLIPR Tetra | Agonist | Antagonist | PrecisION Cell line | |
| Potassium Channels | ||||||||
| Kv1.1 | CYL6014MPC CYL6014MPCDR |
CYL8014QP2 CYL8014QP2DR |
– | – | – | – | – | CYL3014 |
| Kv1.2 | CYL6015MPC CYL6015MPCDR |
– | – | – | – | – | – | CYL3015 |
| Kv1.3 | CYL6016MPC CYL6016MPCDR |
CYL8016QP2 CYL8016QP2DR |
– | – | – | – | – | CYL3016 |
| Kv1.4 | CYL6017MPC CYL6017MPCDR |
– | – | – | – | – | – | CYL3017 |
| Kv1.5 | CYL5018MPC CYL5018MPCDR |
CYL8018QP2 CYL8018QP2DR |
– | – | – | – | – | CYL3018 |
| Kv1.6 | CYL6019MPC CYL6019MPCDR |
– | – | – | – | – | – | CYL3019 |
| Kv1.7 | CYL6020MPC CYL6020MPCDR |
– | – | – | – | – | – | CYL3020 |
| Kv1.8 | CYL6021MPC CYL6021MPCDR |
– | – | – | – | – | – | CYL3021 |
| Kv2.1 | CYL6022MPC CYL6022MPCDR |
– | – | – | – | – | – | CYL3022 |
| Kv2.1/Kv9.2 | CYL6067MPC CYL6067MPCDR |
– | – | – | – | – | – | CYL3067 |
| Kv3.1 | CYL6043MPC CYL6043MPCDR |
– | – | – | – | – | – | CYL3043 |
| Kv3.2 | CYL6044MPC CYL6044MPCDR |
– | – | – | – | – | – | CYL3044 |
| Kv3.3 | CYL6045MPC CYL6045MPCDR |
– | – | – | – | – | – | CYL3045 |
| Kv3.4 | CYL6046MPC CYL6046MPCDR |
– | – | – | – | – | – | Inquire |
| Kv4.2/KChIP2 | CYL6026MPC CYL6026MPCDR |
– | – | – | – | – | – | CYL3026 |
| Kv4.3/KChIP1 | CYL6027MPC CYL6027MPCDR |
– | – | – | – | – | – | CYL3027 |
| Kv4.3/KChIP2 | CYL5069MPC CYL5069MPCDR |
CYL8069QP2 CYL8069QP2DR |
– | – | – | – | – | CYL3069 |
| Kv7.2/Kv7.3 | – | – | CYL8059QB CYL8059QBDR CYL8059QB2C CYL8059QB2DR CYL8059QB3 |
– | – | – | – | CYL3059 |
| Kv7.3/Kv7.5 | CYL6060MPCDR | – | – | – | – | – | – | CYL3060 |
| Kv7.4 | CYL6092MPCDR | – | – | – | – | – | – | CYL3092 |
| Kv7.4/Kv7.5 | CYL6096MPCDR | – | – | – | – | – | – | CYL3096 |
| Kv11.1 hERG-CHO | – | CYL8038QP2 CYL8038QP2DR |
CYL8038QB2 CYL8038QB2DR |
– | – | – | – | CYL3038 |
| Kv11.1 hERG-HEK | CYL5039MPC | – | – | – | – | – | CYL3039 | |
| Kv12.2 | CYL6089MPC | – | – | – | – | – | – | CYL3089 |
| Kir2.1 | CYL5032MPC | – | – | – | – | – | – | CYL3032 |
| Kir 6.2/ SUR 2A | CYL6099MPCDR | CYL8099QP1 CYL8099QP1DR |
– | Inquire | – | – | – | CYL3099 |
| KCNQ1/hminK | CYL5007MPC | – | – | – | – | – | – | CYL3007 |
| Sodium Channels | ||||||||
| Nav1.1 | CYL6009MPC CYL6009MPCDR |
CYL8009QP2 CYL8009QP2DR |
– | – | – | – | – | CYL3009 |
| Nav1.2 | CYL6023MPC CYL6023MPCDR |
CYL8023QP2 CYL8023QP2DR |
– | – | – | – | – | CYL3023 |
| Nav1.3 | CYL6003MPC CYL6003MPCDR |
CYL8003QP2 CYL8003QP2DR |
– | – | – | – | – | CYL3003 |
| Nav1.4 | CYL6024MPC CYL6024MPCDR |
CYL8024QP2 CYL8024QP2DR |
– | – | – | – | – | CYL3024 |
| Nav1.5 | CYL5004MPC CYL5004MPCDR |
CYL8004QP2 CYL8004QP2DR |
– | – | – | – | – | CYL3004 |
| Nav1.6 | CYL6010MPC CYL6010MPCDR |
CYL8010QP1 CYL8010QP1DR CYL8010QP2 CYL8010QP2DR |
– | – | – | – | – | CYL3010 |
| Nav1.7 | CYL6011MPC CYL6011MPCDR |
CYL8011QP2 CYL8011QP2DR |
– | – | – | – | – | CYL3011 |
| Nav1.8/β1 | CYL6025MPC CYL6025MPCDR |
CYL8025QP2 CYL8011QP2DR |
– | – | – | – | – | CYL3025 |
| Nav1.8 (rat) | CYL6050MPC CYL6050MPCDR |
CYL8050QP2 CYL8011QP2DR |
– | – | – | – | – | CYL3050 |
| Calcuim Channels | ||||||||
| Cav1.2 (L-type) | CYL5051MPC CYL5051MPCDR |
CYL8051QP2 CYL8051QP2DR |
– | – | – | – | – | – |
| Cav1.2 (N-type) | CYL6054MPC CYL6054MPCDR |
CYL8054QP2 CYL8054QP2DR |
– | – | – | – | – | CYL3054 |
| Cav3.2 (T-type) | CYL6075MPC CYL6075MPCDR |
CYL8075QP2 CYL8075QP2DR |
– | – | – | – | – | CYL3075 |
| Cav2.2 (N-type) | – | – | – | – | – | – | 216000 216010 216020 164 |
– |
| Ligand-gated Ion Channels – GABA | ||||||||
| GABAA α1/β2/γ2 | – | – | – | CYL8108IF CYL8108IF2 CYL8108IF3 |
– | 4525 | 4518 3051 |
– |
| GABAA α1/β3/γ2 | – | – | – | CYL8053IF CYL8053IF2 CYL8053IF3 |
– | – | – | CYL3053 |
| GABAA α2/β3/γ2 | – | – | – | CYL8072IF CYL8072IF2 CYL8072IF3 |
– | – | – | – |
| GABAA α3/β3/γ2 | – | – | – | CYL8068IF CYL8068IF2 CYL8068IF3 |
– | – | – | CYL3068 |
| GABAA α4/β3/γ2 | – | – | – | CYL8085IF CYL8085IF2 CYL8085IF3 |
– | – | – | CYL3085 |
| GABAA α5/β3/γ2 | – | – | – | CYL8073IF CYL8073IF2 CYL8073IF3 |
– | – | – | CYL3073 |
| GABAA α6/β3/γ2 | – | – | – | CYL8068IF CYL8068IF2 CYL8068IF3 |
– | – | – | CYL3086 |
| GABAA (non-selective) |
– | – | – | – | – | 28, 57 226500 226600 |
170, 3817 226810 |
– |
| Ligand-gated Ion Channels – Acetylcholine Nicotinic Channels | ||||||||
| nAChR α1/β1/δ/ε | – | – | – | CYL8052IF CYL8052IF2 CYL8052IF3 |
– | – | 258700 | CYL3052 |
| nAChR α3/β4 | – | – | – | CYL8057IF CYL8057IF2 CYL8057IF3 |
– | 258730 | – | CYL3057 |
| nAChR α4/α6/β2 | – | – | – | CYL8106IF CYL8106IF2 CYL8106IF3 |
– | – | – | CYL3106 |
| nAChR α4/β2 | – | – | – | CYL8107IF CYL8107IF2 CYL8107IF3 |
– | 3029 299031 258750 |
– | CYL3106 |
| nAChR α7/ric3 | – | – | – | CYL8097IF CYL8097IF2 CYL8097IF3 |
– | – | 3010 299032 258630 |
CYL3097 |
| Ligand-gated Ion Channels – Glycine Receptor | ||||||||
| GlyRA1 | – | – | – | CYL8056IF CYL8056IF2 |
– | – | – | CYL3056 |
| Ligand-gated Ion Channels – 5-HT3 | ||||||||
| 5-HT3 | – | – | – | CYL8109IF CYL8109IF2 |
– | – | 411 271910 |
– |
| TRP | ||||||||
| TRPA1 | – | – | – | Inquire | CYL8066FL CYL8066FL2 CYL8066FL3 |
– | – | CYL3066 |
| TRPM8 | – | – | – | Inquire | Inquire | – | – | |
| TRPV1 | – | – | – | Inquire | CYL8063FL CYL8063FL2 CYL8063FL3 |
– | – | CYL3063 |
| TRPV3 | – | – | – | Inquire | CYL8065FL CYL8065FL2 CYL8065FL3 |
– | – | CYL3065 |
| TRPV4 | – | – | – | Inquire | CYL8064FL CYL8064FL2 CYL8064FL3 |
– | – | CYL3064 |
| Acid-sensing Ion Channel | ||||||||
| ASIC3 | – | – | – | – | CYL8055IF CYL8055IF2 |
– | – | CYL3055 |
| Cystic Fibrosis Transmembrane Conductance Regulator | ||||||||
| CFRT | CYL8088MPC CYL8088MPCDR |
– | – | – | Inquire | – | – | CYL3088 |
| Hyperpolarization-activated, Cyclic Nucleotide-gated Channels | ||||||||
| HCN1 | CYL6040MPC CYL6040MPCDR |
– | CYL8040QB2 CYL8040QB2DR |
– | Inquire | – | – | CYL3040 |
| HCN2 | CYL6041MPC CYL6041MPCDR |
– | CYL8041QB2 CYL8041QB2DR |
– | Inquire | – | – | CYL3041 |
| HCN3 | CYL6042MPC CYL6042MPCDR |
– | CYL8012QB2 CYL8012QB2DR |
– | Inquire | – | – | CYL3042 |
| HCN4 | CYL5012MPC CYL5012MPCDR |
– | – | – | Inquire | – | – | CYL3012 |
| etc. | ||||||||
| IP3R1 | – | – | – | – | – | 242500 | – | – |
| KATP | – | – | – | – | – | – | 165 265600 |
– |
| P2X (non-selective) |
– | – | – | – | – | 127 299036 |
– | – |
| RY2 | – | – | – | – | – | 3800 | – | – |
| RY3 | – | – | – | – | – | 270300 | – | – |
서비스 개요 및 샘플 준비 방법
| Platform | Manual Patch Clamp | Automated Patch Clamp | Binding assay (Agonist / Antagonist) |
||
| QPatch | Qube | IonFlux | |||
| 농도 개수 및 반복 횟수 | 1 농도 (n=3) 5 농도* (n=2) |
3 농도 (n=2) 5 농도* (n=2) |
1 농도 6 농도* |
최소 well: 12 | 1농도 >5 농도* |
| Sample Volume-Liquid (in 100% DMSO) |
300x or 10 mM stock 최소 250 ul |
300x or 10 mM stock 최소 150 ul |
60 µl of 10 mM stock (90 ul for dose-response) |
||
| Sample Volume-Solid | 1 mg 이상 | ||||
| Turnaround Time | 25 days | 15 days | 15 days | ||
| 서비스 장소 | USA – St Charles, MO | Cerep, Taiwan | |||
* Dose-response curve 및 IC/EC50 calculation 제공
서비스 절차
서비스 문의