
Milo™ System
Single-Cell Western platform
- 1,000 개 이상의 단일 세포에 대해 Western 기반의 단백질 분석
- 단 1 mL의 시료로 Protein expression heterogeneity 분석과 세포 내 Subpopulation 확인
- 최대 4 개의 단백질 표적에 대한 Multiplexing 가능
- Flow cytometry 에서는 분석이 어려운 Intracellular protein (Phosphorylated target, Transcription factor, and Protein isoform) 검출 가능
1,000 개 이상의 단일 세포에 대해 Western 기반의 단백질 분석
단 1 mL ( >104 Cells) 의 시료로 Protein expression heterogeneity 분석과 세포 내 Subpopulation 확인
FACS 에서는 분석이 어려운 Intracellular protein (Phosphorylated target, Transcription factor) 과 Protein isoform 검출 가능
최대 4 개의 단백질 표적에 대한 Multiplexing 가능
빠르고 간단한 분석 과정 (Total workflow: 4-6 hours, Hands-on time: 1-2 hrs)
Target expression heterogeneity of tumors
Identify differentiated stem cell subtypes
Intracellular signaling pathway activation
Validating single cell RNA-seq data
[Application Note]
Protein Expression Heterogeneity with Milo, the First Single-Cell Western System >>
Adapting the Single-Cell Western Protocol to Detect Histone Modifications >>
Using Single-Cell Westerns to Validate Single-Cell RNA-Seq Data >>
Identify and Quantify Neural Subtypes with Single-Cell Westerns >>
Description | Specification |
Sample volume required | 1 mL |
Sample type | Suspension containing >10,000 cells |
Cell diameter | 7-25 µm |
Cell type | Mammalian cells; globular in suspension and unfixed |
Antibody requirement | Standard, commercial unlabeled primaries and fluorescent secondaries |
Other equipment needed | Open-format fluorescence microarray scanner capable of 10 µm resolution |
Molecular weight (MW) range | 15-175 kDa |
MW resolution | 10% differences in distinct spectral channels, as low as 30% differences in same spectral channel |
Typical target multiplexing | Up to 4 proteins per cell |
Dynamic range | 3 logs |
Quantitation CV | < 6% |