Megaruptor 2
Long read sequencing을 위한 DNA shearing 장비
- 3kb부터 75kb까지 원하는 사이즈로 DNA shearing 가능
- target size fragment의 distribution이 높음
- PacBio®, Oxford Nanopore®, Illumina®, Ion TorrentTM 플랫폼에 적합
- Tubing 막힘 현상을 완화하고 컴퓨터를 내장한 NEW 모델
- 2개의 시료를 연속적으로 처리
- Cross-contamination 방지
NGS 연구 관련, long reads sequencing을 위한 DNA 절편화 장비
기본적으로 3Kb~75Kb 크기의 DNA fragment 획득 가능
증명된 Long DNA fragmentation 퀄리티
2개의 시료를 연속적으로 처리 가능하며, recalibration이 필요 없음
Syringe pump를 이용하여 DNA shearing
일화용품 사용으로 Cross contamination 제거
간편한 프로그램 및 재현성 있는 결과 제공
Validation using two different DNA sources and two different methods of analysis. A: Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. B: Fragment Analyzer profiles of human genomic DNA (25 ng/μl; 200 μl/sample) sheared at different software settings of 2, 3, 5 and 8 kb. (Standard Sensitivity Large Fragment Analysis Kit ; Advanced Analytical Technologies, Inc. was used for separation and fragment sizing).
- Cell Presence
- Cell Confluence
- Cell Viability Detection
- Live Cell Imaging
- Cell Invasion
- Cell Migration
- Fluorescence Protein Expression
- Cell-based Assay Optimization
| Description | Specification |
| Power Supply | 24V, 3A [Internal] |
| Transformer 220V/110V | Internal |
| Main Unit Dimensions | 67(w) x 218(d) x 255(h) mm |
| Base dimensions [footprint] | 125(w) x 185(d) x 12(h) mm |
| Fragment Length Range Achievable | 2 kb ~ 75 kb |
| Sample Volumes Accepted | Minimum : 50 ul, Maximum: 400 ul [depending on fragment length; see “Processing Samples” chapter] Recommended : 200 ul |
| Sample Concentration | 0 ~ 50 ng/ul, Recommended : 200 ng/ul |
| Tubes | 0.5 ml free-standing, provided |
| Throughtput | Two samples processed in series |
| Time per sample | 10 ~ 20 min [depending on target size]; includes sample processing and system washing |
| Total Weight | 8 kg |
| Control Software | Windows compatible, pre-installed on included taptop |