IP-Star® Compact

Chromatin IP와 NGS library preparation 과정을 자동화하는 장비

Magnetic-bead 기술을 이용한 높은 재현성 있는 결과

Chromatin IP와 NGS prep library과정을 하나의 프로토콜에서 지원

다양한 시약을 자동화할 수 있는 Open platform

터치 방식의 간단한 사용

한 프로토콜에 16 개 시료까지 작동

시료 온도 조절 가능

[Katia Basso at Columbia University – Transcription Factor ChIP-seq with 10 times less input]
• TF ChIP-seq using 10 times less input than a standard ChIP-seq
• High reproducibility of transcription factor ChIP-seq from only 1,000,000 cells
• The simplicity of the 3-step MicroPlex Kit protocol with NO intermediate purifications
• Walk away automation with the IP-Star®

BCL6 binding on CD69 promoter. B cell lymphoma cell line P3HR1 was crosslinked and then sonicated using the Bioruptor® Pico. ChIP was performed on the IP-Star® Automation instrument. The MicroPlex v2 Kit from Diagenode was used for library preparation. The plots represent raw reads, the squares below the plots mark the significantly enriched peaks (according to the peak call software). Red box: peak regions that have been previously demonstrated by independent assays to be enriched for BCL6 binding. Data courtesy of Katia Basso, Columbia University, New York.