GingisREX® (Rgp) Protease
Arginine 잔기의 C-terminal 부위를 특이적으로 가수분해 하는 효소로서,
Arg-C (Clostripain) 와는 달리, Lysine 잔기에는 반응하지 않습니다.
Unique specificity for Arginine residues
Also Active on Arg-Pro linkages
60 min reaction time
Active in 6M urea and at a broad pH range of 5.5-9.0
Applications
– Peptide mapping
– Protein fingerprinting
– Post-translational modification analysis
Unit Definition
GingisREX is a cysteine protease that specifically digests peptide bonds C-terminally to arginine residues, including arginine linked to proline. Cysteine is required for enzymatic activity.
Buffers and Reducing Agent
To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment (for details please consult the user instructions). GingisREX is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween and SDC.
Buffers tested and compatible with GingisREX activity are Tris, Bis-Tris and ammonium bicarbonate.
Storage
GingisREX is shipped cold and should be stored at -20°C upon arrival.
After reconstitution by addition of water, GingisREX is stable for at least 1 month at 4-8°C.
Brochure
Product protocol
| Part number | Item name | Enzyme:Protein Ratio | Description |
| GVB0-GRX-005 | GingisREX, 5 μg enzyme | 1:20 – 1:200 | Lyophilized |