GingisKHAN® (Kgp) Protease
항체의약품의 특성 분석을 위한 Lysine-specific cysteine protease 로서,
Hinge 위를 빠르고 정확하게 잘라 Fab, Fc fragments를 획득할 수 있습니다.
Specific – single amino acid position digestion site in the upper hinge
Rapid – a 60 minute protocol for complete digestion
Intact Fab and Fc fragments from Therapeutic mAbs
Easy to use, no optimization of reaction conditions is needed
별도의 Reducing agent 를 구매할 필요 없이, 함께 동봉 되어있는 GingisKHAN 전용 Reducing Agent (5 Vial)를 사용하시면 됩니다.
Applications
– Generation of Fab fragments from human IgG1
– Subunit LC-MS analysis
– Characterize heavy and light chain pairing of bispecific antibodies
– Study monovalent binding of Fab fragments to the antigen
– Epitope mapping using HDX-MS
– Cystallization of Fab fragments
Unit Definition
One unit GingisKHAN digests ≥ 95% of 1 μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1 h.
Preparations
Prepare the IgG (human IgG1) in digestion buffer. The final IgG concentration should be 0.5-5 mg/ml. GingisKHAN Reducing Agent must be prepared and used the same day, it cannot be stored.
Storage
GingisKHAN and Reducing Agent are shipped cold and should be stored at -20°C upon arrival.
After reconstitution, GingisKHAN is stable for 2 months at +4-8°C.
Brochure
Product protocol
| Part number | Item name | Digestion | Description |
| GVB0-GKH-020 | GingisKHAN® 2000 units | 2 mg hIgG1 | Lyophilized |
| GVB0-GFK-020 | GingisKHAN® Fab kit | 2 mg hIgG1 | Purification |