GalNAcEXO™ (α-GalNAcase)
O-glycosylated protein의 Ser/Thr 잔기에 연결된
α-linked N-acetylgalactosamines (GalNAcs)를 가수분해하는 효소
O-glycosylated protein의 Ser/Thr 잔기에 연결된 α-linked GalNAcs (Tn Antigen) 에 대해 높은 효율로 가수분해함.
Mass Spec 분석에 적합
타사 제품과는 달리 BSA같은 Co-factor가 필요하지 않습니다.
Table 1. Summary of key specifications
Microspin column format으로 활용
GalNAcEXO 효소가 Agarose bead에 공유결합 되어있는 Micro-spin column 형태로 제공됩니다.
원심분리를 통해 잔여효소 없이 Digestion 된 단백질을 얻을 수 있습니다.
Applications:
– Tn antigen characterization on O-glycosylated biopharmaceuticals
– Deglycosylation of theC1 Inhibitor
– Performance testing on Etanercept
Unit Definition
One unit of GalNAcEXO catalyzes the hydrolysis of α-linked GalNAc residues from ≥ 95% of 2 nmol 4-Nitrophenyl 2-acetamido-2-deoxy-D-galactopyranoside when incubated in 20 mM Tris, 1 % ethanol pH 6.8 at 37°C for 30 minutes, as monitored by reverse phase chromatography.
Contents
GalNAcEXO enzyme is supplied as a lyophilized powder in Tris buffer saline (TBS) pH 7.6, with no preservatives added.
Storage
GalNAcEXO is shipped cold and should be stored at -20°C upon arrival.
After reconstitution GalNAcEXO is stable for at least 1 month at +4-8 °C.
| Part number | Item name | Digestion | Description |
| GVG1-NA1-020 | GalNAcEXO, 2000 units | 2 mg | Lyophilized |
| GVG1-NA6-025 | Immobilized GalNAcEXO Microspin | 5 x 0.5 mg | Microspin column format |
| GVG1-NA6-050 | Immobilized GalNAcEXO Microspin | 10 x 0.5 mg | Microspin column format |