FabULOUS® (SpeB) Protease
Human 뿐 아니라 Mouse, Rat, Goat, Sheep, Rabbit 등 다양한 종의 IgG에 반응하며,
Hinge 위(THT/CPP)를 신속 정확하게 잘라 Fab, Fc fragments를 획득할 수 있습니다.
Broad species range activity, including mouse IgG1
Requires reducing conditions
1.5 mM DTT, 1-5 mM TCEP, 50-100 mM cysteime, 50-100 mM 2-mercaptoethanol
60 min reaction time
FabULOUS Fab Kit generates and purifies Fab fragments from mouse IgG
FabULOUS Fab kit consists of 2000 units of the FabULOUS enzyme and 4 x CaptureSelect™* LC-kappa affinity spin columns for generation and purification of Fab fragments from mouse IgG
Applications
– Study monovalent binding of Fab fragments to the antigen
– Elimination of Fc-mediated effector functions
– Reduced unspecific binding from Fc interactions
Unit Definition
One unit digests ≥ 95% of 1 µg human IgG1 when incubated in PBS with 5 mM DTT or TCEP, pH 7.4 at 37°C for 1 hour.
Buffers and Reducing Agent
FabULOUS digests IgG in commonly used buffers with pH ranging from 6.5 to 8.0 and best activity is obtained at 37°C. The enzyme requires reducing agent in the digestion reaction and depending on the reducing conditions, there is a chance that the interchain thiols will be reduced. Buffers tested and compatible with GingisREX activity are Tris, Bis-Tris and ammonium bicarbonate.
Storage
The FabULOUS enzyme is shipped cold and should be stored at -20°C upon arrival.
After reconstitution, FabULOUS is stable for at least 1 month at +4-8°C.
Brochure
Product protocol
| Part number | Item name | Digestion | Description |
| GVA0-PU1-020 | FabULOUS, 2000 units | 2 mg IgG | Lyophilized |
| GVA0-PFK-020 | FabULOUS Fab kit mouse,
2000 units |
2 mg mIgG | For generation and purification of Fab fragments from mouse IgG. |