FabALACTICA™ (IgdE) Protease
항체 의약품의 특성 분석을 위한 Human IgG1-specific protease 로서,
Hinge 위를 빠르고 정확하게 잘라 Fab, Fc fragments를 획득할 수 있습니다.
High specificity for digestion above the hinge of human IgG1
Intact Fab and Fc fragments from Therapeutic mAbs
No need for reducing agents or co-factors
FabALACTICA Fab kit – Platform method for Fab preparation
FabALACTICA Fab Kit 제품은 FabALACTICA가 고정된 Spin column 과 Fc fragments와 친화적으로 결합하는 CaptureSelect™ Fc resin column으로 구성 되어있습니다. Digestion후에 Fc fragments는 CaptureSelect column과 결합하고, 순수한 Fab fragments 만 획득 가능합니다.
Applications
– Subunit LC/MS analysis
– Paired glycan analysis
– Characterization of bi- or multispecific antibodies
– Affinity screening
– Studies of higher order structures
– Studies on antibodies with mutated hinges
Unit Definition
One unit FabALACTICA digests ≥ 90 % of 1 μg human IgG1, when incubated in 150 mM sodium phosphate, pH 7.0 at 37°C overnight (16-18 hour)
FabALACTICA is derived from Streptococcus agalactiae and expressed in E. coli.
The enzyme contains a His-tag and the molecular weight is 70 kDa.
Digestion site
KSCDKT / HTCPPCP on human IgG1
Storage
FabALACTICA is shipped cold and should be stored at -20°C upon arrival.
After reconstitution, the FabALACTICA enzyme is stable for at least 1 month at +4-8°C.
| Part number | Item name | Digestion | Description |
| GVA0-AG1-020 | FabALAItem nameCTICA, 2000 units | 2 mg hIgG | Lyophilized |
| GVA0-AG6-010 | Immobilized FabALACTICA Microspin | 2 x 0.5 mg hIgG1 | Spin columns are provided with immobilized FabALACTICA on a agarose bead |
| GVA0-AG6-050 | 10 x 0.5 mg hIgG1 | ||
| GVA0-AG6-100 | Immobilized FabALACTICA Midispin | 5-10 mg hIgG1 | |
| GVA0-AG6-1000 | Immobilized FabALACTICA Maxispin | 10-100 mg hIgG1 | |
| GVA2-AFK-005 | FabALACTICA Fab kit | 0.5 mg hIgG1 | Easy generation and purification of Fab fragments from human IgG1 antibodies |
| GVA2-AFK-025 | 5 x 0.5 mg hIgG1 | ||
| GVA2-AFK-100 | 10 mg hIgG1 | ||
| GVA2-AFK-1000 | 100 mg hIgG1 |