3D CoSeedis
– 3개의 cell type까지 함께 실험 및 분석이 가능한 3차원 세포 배양 gel
– Tissue specific한 ECM의 환경 구현 가능
– Serum-reduced, serum-free 환경 구현 가능
Modular set-up 가능
– 3개의 cell type까지 함께 분석 가능 / tissue specific ECM 구현
– Contact or distance co-culture with feeder cells
– Serum, serum-reduced, serum-free 환경 구현
Agarose-based Permeable Matrix로 구성
– 3차원 세포의 long-term culture
– 양분, 산소 등의 충분한 공급 & 독성 분해 물질의 제거
– Feeder cell 및 particles와 co-culture 가능
– Chip 속의 3D cell aggregates에 growth (or other) factors를 diffusion을 통해 제공 가능
Distance co-culture
– Feeder cells의 완전하게 분리된 배양 가능 (자체 특허 기술): feeder cell과 함께 배양 시 spheroid가 생존도가 영향 받는 단점을 보완
– 대부분의 primary cell과 iPS에 적용 가능
다양한 application
– 다양한 cell type에 응용
– Spheroid 뿐만 아니라 and non-spheroidal aggregates도 배양 가능
– high-throughput and high-content 적용
– 3D cell constructs의 높은 재현성 (Reproducibility)
Three-dimensional cell models for extracellular vesicles production, isolation, and characterization
Liliia Paniushkina, Elena Grueso-Navarro, Xu Cheng, Irina Nazarenko [자세히 보기]
Tspan8 is expressed in breast cancer and regulates E-cadherin / catenin signalling and metastasis accompanied by increased circulating extracellular vesicles
Maren Vogelstaetter et al., J. Pathol., 2019 [자세히 보기]
3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles
Sara Rocha et al., Adv. Sci. 2018, 1800948 [자세히 보기]
A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement
Andreas R. Thomsen et al., Lab Chip, 2018, 18, 179 [자세히 보기]
Proteome Profiling of Primary Pancreatic Ductal Adenocarcinomas Undergoing Additive Chemoradiation Link ALDH1A1 to Early Local Recurrence and Chemoradiation Resistance
V. O. Oria et al., Translational Oncology, Vol. 11, Issue 6, Dec. 2018 [자세히 보기]
A binary approach to the colony forming assay: reliable and reproducible read-outs using 3D CoSeedis™
Marco P. Leu et al. [자세히 보기]
Easy mass production of homogenous and uniform 3D spheroids for high-throughput screening applications
Marco P. Leu et al. [자세히 보기]