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StemFit iPSC/ESC Culture Media
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StemFit iPSC/ESC Culture Media

 

  • 줄기세포 제품의 빠른 승인을 위한 Full Chemically defined media
  • Weekend Free 프로토콜로 비용 절감 가능
  • 고농도 고효율 배지로 높은 생장률 확보
  • 뛰어난 안정성, 배치별 재현성

 

ico_chk01  StemFit iPSC/ESC Culture Media

Product StemFit Basic03 StemFit Basic03 GMP StemFit Basic04 CT
Cat. No. AJBASIC03 AJ34770-10 AJBASIC04CT
Volume 500 mL/bottle 500 mL/bottle 500 mL/bottle
Single-cell culture
Xeno-free
Animal-origin free
Clinical research O (AK03N in Japan) X
cGMP X X
Composition Basal media 400 mL +
Supplement 100 mL (분리)
Basal media 400 mL +
Supplement 100 mL (분리)
Basal media +
Supplement 500 mL (혼합)
bFGF 불포함 불포함 80 ng/mL

 

ico_chk01  경제적인 시스템

Weekend-free feeding을 할 수 있어 기존 방식 대비 medium 40-60%, 비용 30-50% 절감 가능

 

 

ico_chk01  100배 이상의 뛰어난 생장률

여러 matrix에서 기존 방식 대비 100배 이상의 높은 생장률 확보

Human 201B7 iPSCs grown on MEFs (feeder-dependent) were transitioned to feeder-free conditions with StemFit Basic02 or commercially available medium A on respective ECMs (1000 cells/cm2), and cultured for one week.

 

 

ico_chk01  탁월한 colony 생성 능력

높은 cloning efficiency 제공  (Cloning efficiency = Obtained colonies / Seeded cells)

Human iPSCs were adapted to StemFit Basic02, or commercially available medium A or B on Matrigel® for more than 3 passages. Then, cells were serially diluted and seeded with each medium on Matrigel®-coated 96-well plates at 1 cell/well or 10 cells/well. The number of seeded cells was counted after 3 hours, and colonies were counted at day 7.

 

 

ico_chk01  안정성 및 재현성이 높은 feeder-free 배양 시스템

 

ico_chk01  Selection Guide

ico_chk01 StemFit For Differentiation

hPSC 확장을 위해 StemFit 배지와 결합하면 hPSC 확장 및 분화 모두에 CD-AOF 배양 시스템을 제공함으로써 hPSC 유래 세포 / 조직의 임상 적용이 가능합니다. Diff에 대한 StemFit. 5x 농도로 제공되며 배지 (DMEM / F12, RPMI1640, DMEM 등) 및 다양한 유도 인자 또는 사이토 카인과 함께 사용 할 수 있습니다.

 

 

ico_chk01 StemFit for MSC

동물 유래 성분 없이 Recombinant protein 으로만 제작된 MSC media 는 추후 임상에 적용을 목적으로 할 시, 성분의 출처가 확실하여 식약처 및 각종 임상 신청 시 시간 및 비용이 줄어듭니다.

 

 

ico_chk01 Growth Factor

ⓐ bFGF

FGF 염기성 (bFGF)은 사이토 카인의 FGF 패밀리의 구성원이며 많은 유형의 세포의 증식, 뉴런 분화 및 다 능성 상태의 유지를 포함하여 광범위한 생물학적 과정에 관여합니다. bFGF는 다능성 줄기세포 또는 중간엽줄기 세포를 확장시키기 위해 주로 줄기 세포 배양에 사용됩니다. 인간 bFGF 재조합 체는 154 개의 아미노산 잔기를 함유하는 17.1 kDa입니다.

 

 

ⓑ ActivinA

Activin A는 사이토카인의 TGF-베타 수퍼패밀리이며 조직 형태 형성 및 복구, 섬유증, 염증, 신경 발달, 조혈, 생식계 기능 및 발암을 포함한 광범위한 생물학적 과정에 관여합니다. Human activin A는 각각 116 개의 아미노산 잔기를 함유하는 2 개의 βA chain의 26.0 kDa disulfied 연결된 homodimer입니다. activin A는 주로 줄기 세포를 내배엽 또는 중배엽으로 분화시키기 위해 줄기 세포 배양에 사용됩니다.

ico_chk01  Development for StemFit media
Sci.Rep. 2014 Jan 8;4:3594.
A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.

 

ico_chk01  Maintenance protocol for hPSCs with StemFit Basic02 medium
Nat Protoc. 2017 Jan;12(1):195-207.
Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.

Curr Protoc Stem Cell Biol. 2018 e50
CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.

 

ico_chk01  Application for genome-edited clone generation
Methods. 2016 May 15;101:43-55.
Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.

Stem Cell Reports. 2016 Apr 12;6(4):496-510.
Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells.

Hum Mol Genet. 2016 Oct 18.
Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders.

Curr Protoc Stem Cell Biol. 2018 e50
CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.

 

ico_chk01  Liver & Gut organoids

Cell Rep. 2017 Dec 5;21(10):2661-2670.
Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells.

Nature. 2017 Jun 22;546(7659):533-538.
Multilineage communication regulates human liver bud development from pluripotency.

Stem Cell Reports. 2018 Mar 13;10(3):780-793.
Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids.

 

ico_chk01  Kidney organoids
Cell Stem Cell. 2017 Dec 7;21(6):730-746.e6.
Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.

Nat Protoc. 2017 Jan;12(1):195-207.
Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.

Methods Mol Biol. 2017;1597:179-193.
Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells.

 

ico_chk01  Differentiation
PLoS One. 2014 Dec 2;9(12):e112291.
Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media.

Stem Cell Res. 2017 Dec;25:98-106.
A human iPS cell myogenic differentiation system permitting high-throughput drug screening.

Stem Cell Reports. 2017 Nov 14;9(5):1406-1414.
Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.

Stem Cell Reports. 2016 Mar 8;6(3):312-20.
Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates.

Nature. 2017 Aug 30;548(7669):592-596.
Human iPS cell-derived dopaminergic neurons function in a primate Parkinson’s disease model.

Mol Brain. 2016 Sep 19;9(1):85.
Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases.

 

ico_chk01 TIP

Single-cell cloning with coating-free method 다운로드

Successful single-cell passage 다운로드

 

ico_chk01 프로토콜 비디오

Establishment of iPS cells by the episomal method

(1) Preparation of plates

(2) establishment by the episomal method

 

Passage method

(1) Preparation of plates and medium

(2) Passage

 

Freezing

(1) Preparation of medium and detachment of cells

(2) Cell freezing