OmniGLYZOR
• N- and simple mucin-type O-glycans on glycoproteins
• 1-4 hour reaction
• RapiGest SF and lyophilized PNGase F are included
• Hydrolysis of N- and O-glycans from glycoproteins
Reagents included in OmniGLYZOR:
• OmniGLYZOR Microspin columns (5 x 50-100 μg or 10 x 50-100 μg)
• Lyophilized PNGase F (1000 units, 1 vial)
• RapiGest SF Surfactant (1 mg, 1 or 2 vials)
OmniGLYZOR Microspin columns contain sufficient material to remove N- and O- glycans from 50-100 μg glycoprotein. The resin is supplied in 20% EtOH with no preservatives added.
OmniGLYZOR Microspin columns are shipped cold and should be stored at +4-8°C upon arrival. Do not freeze the microspin columns!
PNGase F is supplied lyophilized in 50 mM HEPES buffer pH 7.5, with no preservatives added. PNGase F is shipped cold and should be stored at -20°C upon arrival. After reconstitution, PNGase F is stable for at least 1 month at +4-8°C. RapiGest SF* should be stored at room temperature. Once reconstituted in high purity water or a buffer (pH 7–10), the solution is stable for one week when stored at +2–8°C. OmniGLYZOR is for R&D use only.
* RapiGest™ SF Surfactant is a trademark of Waters Corporation.
Part number | Item name | Description |
G3-OM6-005 | OmniGLYZOR, Microspin 5 x 50-100 μg | Deglycosylates 5 x 50-100 μg glycoprotein |
G3-OM6-010 | OmniGLYZOR, Microspin 10 x 50-100 μg | Deglycosylates 10 x 50-100 μg glycoprotein |