O-Glycans
항체신약 개발분야에서 만들어진 항체를 확인할 때 O-glycan 분석에 적용할 수 있는 enzyme 입니다. 이중에서 3가지가 상용화 되었습니다.
– OpeRATOR
– OglyZOR
– SialEXO
정확하게 하나의 특정 부위만을 자를 수 있음
Enzyme에 대한 optimization 과정이 필요 없음
100% 가까운 yield를 얻을 수 있음
반응시간 30분으로 단축 할 수 있음
OpeRATOR™ is an O-glycan specific protease that digestsO-glycosylated proteins N-terminally of the S/T glycosylation site.
- Glycan specific endoprotease
- Digests N-terminally of O-glycans, at serine or threonine residues
- Requires O-glycans for activity – no activity on glycoproteins with only N-glycans
- Increased performance when pretreated with SialEXO
OglyZOR™ is an endoglycosidase that specifically hydrolyzes core 1 and core 3 O-glycans on native glycoproteins. It requires removal of sialic aids for activity, and is provided together with SialEXO™ (sialidase).
- Endoglycosidase acting on O-glycans
- Active on native glycoproteins
- Specific for core 1 and core 3 O-glycan disaccharides
- Requires pretreatment with SialEX
SialEXO™ is a sialidase mix for complete removal of sialic acids from native glycoproteins.
- Acts on native glycoproteins
- Cleaves α2-3, α2-6 and α2-8-linked sialic acids
- Acts on both N- and O-linked glycans
- Hydrolyzes sialic acids within 2 h
- Compatible with OpeRATOR and OglyZOR
- Glycan profiling
- Glycan site determination
- Middle-down approaches for O-glycosylated proteins
- Removal of O-glycans for glycan analysis
- Confirmation of O-glycan presence
- Reduction of sample heterogeneity
- Removal of all sialic acids
- Pretreatment prior to OpeRATOR incubation
- Pretreatment prior to OglyZOR deglycosylation
- Exoglycosidase array
