MaxCyte STx
전기충격 방법을 사용하여 세포 내부로 DNA, mRNA, 화합물 등을 고용량, 고효율로 전달 할 수 있는 제품
- Flow electroporation이 가능하여 빠른 속도로 대용량의 transfection이 가능한 모델
- 7.5×10⁴ ~ 2×10¹⁰ cell 까지 적용 가능
- In vivo study 를 위한 stable cell line 빠르게 구축 가능
- 백신 개발, 단백질 대량 생산에 적합
- ISO-certified, and CE-marked
Any Cell: 80여개의 Cell type-specific protocol 내장
Immortalized Mammalian Cells | Hematopoietic Cells | Primary Cells | |
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Mammalian Cancer Cell Lines | Stem Cells | Insect cells | |
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Any Molecule: 다양한 물질의 고용량 Transfection 가능
Any Scale: 최소 75K 부터 최대 2E10 개의 세포까지 scale-up 가능 (Seamless Scalability)
Scale-up 후에도 transfection efficiency가 유지되기 때문에 re-optimization 과정이 불필요하므로 결과물 도출을 위한 시간 절약
다양한 세포에서 95% 이상의 Transfection Efficiency와 90% 이상의 Viability를 보임
Item | Specification |
STx Instrument Dimensions | 8” wide x 19.5” high x 17” deep |
STx Instrument Weight | 55 lbs |
STx Input Power | 100-240VAC, 50-60Hz, 3.5A |
Fuse Requirements | 2X 4A Slow Blow, 250V, 5X20mm |
Operating Humidity | 93% max |
Operating Temperature | 15°C – 25°C |
Storage Temperature | 0°C – 45°C |
Modes of Operation | Static, Flow |
Process Volumes | 15 μL – 100 mL |
Performance (Flow Mode) | 8 mL / minute |
Ports Available | 1 USB / 1 Ethernet |
- Developing Assays for Screening GPCRs with Transiently Transfected Cells Using the MaxCyte® STX™ System [자세히 보기]
- Rapid Production of Cells for Screening Voltage-Gated Ion Channels in Automated Electrophysiology Assays Using the MaxCyte® STX™ Scalable Transfection System [자세히 보기]
- Keys to Successful Cell-Based Assay Development with the MaxCyte® STX™ Scalable Transfection System [자세히 보기]
1. COVID-19 치료제 개발 관련 연구
- X He et al. (2021). Generation of SARS-CoV-2 reporter replicon for high-throughput antiviral screening and testing. Proc Natl Acad Sci U S A 118(15):e2025866118. doi: 10.1073/pnas.2025866118 [자세히 보기]
- BE Jones et al. (2021). The neutralizing antibody, LY-CoV555, protects against SARS-CoV-2 infection in non-human primates. Sci Transl Med Apr 5;eabf1906. doi: 10.1126/scitranslmed.abf1906 [자세히 보기]
2. CRISPR/Cas9 delivery system in iPSC
- Y Iwamoto et al. (2021). Generation of macrophages with altered viral sensitivity from genome-edited rhesus macaque iPSCs to model human disease. Mol Ther Methods Clin Dev 17;21:262-273. doi: 10.1016/j.omtm.2021.03.008. [자세히 보기]
3. Ion Channel
- H Huang et al. (2021). Disease-linked supertrafficking of a potassium channel. J Biol Chem 15;296:100423. doi: 10.1016/j.jbc.2021.100423. [자세히 보기]
- D Simkin et al. (2021). Dyshomeostatic modulation of Ca2+ – activated K+ channels in a human neuronal model of KCNQ2 encephalopathy. Elife 5;10:e64434. doi: 10.7554/eLife.64434. [자세히 보기]
4. GPCR
- Enabling rapid and sensitive GPCR assay development with the MaxCyte ® STX™ Scalable Transfection System and the Codex ACTOne biosensor technology. [자세히 보기]
5. Antibody Production
- K Hayashi et al. (2021). Complex of human Melanotransferrin and SC57.32 Fab fragment reveals novel interdomain arrangement with ferric N‑lobe and open C‑lobe. Sci Rep. 12;11(1):566. doi: 10.1038/s41598-020-79090-8. [자세히 보기]
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