- 4-laser (405nm/488nm/561nm/638nm), 최대12개 형광 분석가능
- Easy to Set up: Fully Automated System, Cleaning Mode 지원
- Flexible Application with 70㎛, 100㎛, 130㎛ chip
- Disposal Microfluidic chip 사용으로 Flow cell과 Nozzle 관리 용이
전 세계의 실험실에서는 면역학, 후성 유전체학, 발달생물학, 신경과학 및 세포 지도 매핑에 이르기까지 생명과학의 여러 영역에 걸쳐 실험 데이터 품질을 개선하기 위해 Sony MA900를 선택하고 있습니다. SONY MA900은 Disposal Microfluidic chip을 사용하여 nozzle clogging Issue 및 교차오염 없이 모든 사용자가 다양한 세포 유형을 쉽게 분류할 수 있으며, 직관적인 소프트웨어는 초보자부터 전문자가까지 모든 연구자가 손쉽게 사용할 수 있도록 합니다.
Maintenance (cleaning) wizard 제공으로 내부오염물질 제거 관리 용이
Disposal microfluidic chip 사용으로 nozzle clogging issue 및 Nozzle 관리 용이
Flow cell 노후로 인한 optical performance 감소 문제 발생하지 않음
Autoclave 가능한 Sheath tank(10L), DW tank(5L), Waste tank(10L)
6 well 부터 384 well까지 간단하게 설정 후 사용 가능, downstream end point assay 및 metadata 분석 가능
Index Sorting 기능을 이용하여 세포주, NGS 실험 최적화 및 응용 가능
Mouse spleen cells were stained with following antibodies: CD4 PerCP-Cy5.5, Alexa Fluor® 700 CD25, and PE CD127. Cells were incubated for 20 minutes on ice, washed 2X with staining buffer, and analyzed on the MA900. Live spleen cells were gated using the scatter gate (A). The CD4+ population (B) was used to gate CD4+CD25int/highCD127low regulatory T cells (C). These cells were sorted using the MA900. Post-sort analysis of the sorted regulatory T cells is shown (D).
Whole blood was lysed with RBC Lysis Buffer and stained with the following antibodies: Alexa Fluor® CD3, PE CD19, PerCP-Cy5.5 CD8, PE/Dazzle 594 CD16, BD Horizon Brilliant Violet 421 (BV421) CCR7, BV510 CD27, BV605 CD20, BV650 CD45RA, BV711 HLA-DR, and BV750 CD4. Cells were incubated for 20 minutes on ice, washed 2X with staining buffer, and analyzed on the MA900. Lymphocytes were gated using the scatter gate (A).
The CD3+ population (B) was used for gating CD4+ and CD8+ cells (C). CCR7+CD45RA+ lymphocytes are shown in (D). CD19+CD20+ B cells were gated from lymphocytes (E), and the HLA-DR expression of B cells was analyzed (F). CD16+ NK cells (G) were gated from lymphocytes. CD27 expression of CD45RA+ subsets of lymphocytes was analyzed (H).
Using the 4-way sort mode on the MA900 cell sorter, CD4+ and CD8+ T cells, CD19+ B cells, and CD16+ NK cells were sorted. Post-sort analysis of each sorted population is shown (G–J).
|Optics||Excitation lasers||4488 nm, 638 nm, 405 nm, 561 nm|
|Output power||Optical fiber output : 405 nm: (10 mW max.), 488 nm, 638 nm, and 561 nm: (36 mW max.)|
|Beam alignment||Dual axis optical system|
|Detection parameters||12 fluorescence + 2 scatter|
|Pulse measurement||Height, Area, Width|
|Fluidics||Sample tube||Single, auto-loading tube|
|Tube types||0.5-mL, 1.5-mL, 5-mL, and 15-mL tubes|
|Sort devices||2-way tube, 4-way tube, multiwell plates, PCR plates|
|Temperature control||5°C, 37°C (electric cooling method)|
|Agitation unit||Eccentric rotation|
|Magnetic drive||300 rpm speed|
|Sorting chip size||70 µm, 100 µm, 130 µm|
|Event rate||70,000 eps|
|Sorting speed||Automated frequency search range
•70 μm : 40 kHz to 52 kHz
•100 μm [Targeted] setting : 21 kHz to 23.5 kHz
•100 μm [Standard] setting : 27 kHz to 31 kHz
•130μm: 10 kHz to 12 kHz
Using the 70-μm sorting chip at 52 kHz and a threshold rate of 12,000 events per second, purity >98% and recovery >80% can be achieved. The yield obtained is based on Poisson’s statistics. Higher threshold events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.
|Scatter resolution||0.5 µm|
|Fluorescence resolution||<3.0% coefficient of variation (CV): PI stained CEN|
|Fluorescence sensitivity||FITC ≤94 MESF, PE ≤88 MESF|
|Ancillary||Dimensions||W: 21.7” (55 cm) x D: 21.7” (55 cm) x H: 28.4” (72 cm)|
|Fluidics cart||W: 33.9” (86 cm) x D: 17.3” (44 cm) x H: 11.8” (30 cm)|
|Weight||231 lb (105 kg)|
|Fluidics cart||108 lb (49 kg) (dry weight)|
|LCD panel||7-inch, 800 x 480 pixels|
|Power supply||100–240 V, 50/60 Hz|
|Power consumption||600 W (max.)|
|Operating temperature||19.5°C to 27.5°C|
|Relative humidity||20% to 80%|
|Compliance||Operating system||Microsoft® Windows® 10 Professional, 64 bit|
|Data file structure||Flow Cytometry Standard (FCS) 3.0 or 3.1|
|Safety standards compliance||UL, CE, CSA|