IgGZERO® (EndoS)
IgG 특이적으로 Complex type의 N-glycan 을 효율적으로 가수분해 하는 탈당화효소(Endoglycosidase)
IgGZERO는 IgG-specific endoglycosidase로서, Complex type의 N-glycan을 가수분해함.
Chitobiose Core에 반응하며, 첫 번째 GalNAc 뒤에서 절단합니다.
타사 비교
Microspin column format으로 활용 (품명: Immobilized IgGZERO® – deGlycIT™)
IgGZERO가 Agarose bead에 공유결합 되어있는 Micro-spin column 형태로 제공됩니다.
원심분리를 통해 잔여효소 없이 Deglycosylation 된 단백질을 얻을 수 있습니다.
반응시간 30분 소요
Applications:
– Rapid assessment of afucosylation levels by LC-MS
– Reduce the complexity of antibody-based therapeutics
– Inactivation of antibodies in immunoassays
Unit Definition
One unit deglycosylates ≥ 95% of 1μg human IgG, when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.
Storage
The IgGZERO enzyme is supplied lyophilized and reconstituted by addition of water. After reconstitution, IgGZERO is stable for 1 month at +4-8°C. The product is shipped at ambient temperature and should be stored at -20°C upon arrival.
| Part number | Item name | Digestion | Description |
| GVA0-IZ1-010 | IgGZERO, 1000 units | 1 mg IgG | Lyophilized powder |
| GVA0-IZ1-050 | IgGZERO, 5000 units | 5 mg IgG | Lyophilized powder |
| GVA0-IZ8-020 | IgGZERO LE (low endotoxin),
2000 units |
2 mg IgG | Cell & Tissue assay, Lyophilized |
| GVA0-IZ6-010 | deGlycIT Microspin | 2 x 0.5 mg | Microspin column format |
| GVA0-IZ6-025 | 5 x 0.5 mg | ||
| GVA0-IZ6-050 | 10 x 0.5 mg | ||
| GVA0-IZ6-100 | deGlycIT Midispin | 1-10 mg | |
| GVA0-IZ6-1000 | deGlycIT Maxispin | 10-100 mg |