GlyCLICK
기존의 Click-chemistry와 달리 Antibody FC region에 Specific 하게 작용하여 높은 수율과 안정된 면역반응의 Conjugation antibody를 얻을 수 있는 Enzyme.
안정성, 면역 반응성 보장
Fc region에만 Specific 하게 작용하여 특정 pH조건에 영향을 받지 않아 안정성, 면역 반응성 보장.
(Lysine, Cystine conjugation의 경우 pH 조건 필요)
다양한 동물의 IgG form 에서 사용 가능
Human IgG뿐 아니라 mouse, rabbit, rat, monkey, sheep, goat, cow, horse 등 다양한 동물의 IgG form 에서 사용 가능
다양한 Labeling application 제공 가능
GlyCLICK works
- Deglycosylation
The Fc specific Endoglycosidase GlycINATOR® (EndoS2)1 hydrolyzes the Fc glycans to the inner most GlcNAc moiety on several subclasses and species of IgG. The enzyme removes all glycoforms, including; high-mannose, hybrid, complex, and bisecting type glycans. - Azide Activation
Azide-containing UPD-GalNAz is enzymatically attached to the exposed GlcNAc using the ß-1,4-Galactosyltransferase Y289L* (GalT) to generate an azide-activated antibody that is reactive with an alkyne-carrying label. - CLICK Reaction
The azide activated antibody is conjugated with a functionalized label2 of choice in a biorthogonal reaction through strain-promoted copper-free click chemistry (SPAAC)3 to form a stable triazole.