FabRICATOR® (IdeS) Protease
항체의약품의 특성 분석을 위한 IgG-specific protease 로서,
Hinge 아래를 빠르고 정확하게 잘라 F(ab’)2 와 Fc/2 fragments를 획득할 수 있습니다.
One specific digestion site below the hinge
30 min reaction
≥95% highly pure fragments
No need for reducing agents or co-factors
Applications
– Antibody Subunit Analysis using HPLC
– Antibody Fc-glycan Analysis by FabRICATOR Digestion and LC-MS
– Determining the Degree of Lysine Clipping
– mAb Oxidation Monitoring Using MS
– High Resolution LC/MS for Amino Acid Sequence Verification
IgG Protease Digestion Chart
Buffers and pH compatible with FabRICATOR digestion
Storage
FabRICATOR is shipped at ambient temperature and should be stored at -20°C upon arrival.
After reconstitution, FabRICATOR is stable for at least 1 month at +4-8°C.
Brochure
Application note
Product protocol
| Part number | Item name | Digestion | Description |
| GVA0-FR1-020 | FabRICATOR, 2000 Units | 2 mg IgG | Lyophilized |
| GVA0-FR1-050 | FabRICATOR, 5000 Units | 5 mg IgG | Lyophilized |
| GVA0-FR1-250 | FabRICATOR, 5×5000 units | 25 mg IgG | Lyophilized |
| GVA0-FR1-008 | FabRICATOR, 8×100 Units | 8 x 100 ug | Lyophilized |
| GVA0-FR1-096 | FabRICATOR, 96×100 Units | 96 x 100 μg | Lyophilized |
| GVA0-FR8-020 | FabRICATOR LE
(Low endotoxin), 2000 units |
2 mg IgG | Cell & Tissue assay, Lyophilized |
| GVA0-FR8-050 | FabRICATOR LE
(Low endotoxin), 5000 units |
5 mg IgG | |
| GVA0-FRZ-020 | FabRICATOR Z, 2000 units | 2 mg
mouse IgG2a |
Lyophilized |
| GVA0-FR6-010 | FragIT Microspin,
(Immobilized FabRICATOR®) |
2 x 0.5 mg | Immobilized FabRICATOR enzyme on agarose beads |
| GVA0-FR6-025 | 5 x 0.5 mg | ||
| GVA0-FR6-050 | 10 x 0.5 mg | ||
| GVA0-FR6-100 | FragIT Midispin | 1-10 mg | |
| GVA0-FR6-1000 | FragIT Maxispin | 10-100 mg | |
| GVA2-FR2-005 | FragIT kit | 0.5 mg IgG | Rapid purification of F(ab’)2 and Fc/2 fragments from IgG |
| GVA2-FR2-025 | 5 x 0.5 mg IgG | ||
| GVA2-FR2-100 | 10 mg IgG | ||
| GVA2-FR2-1000 | 100 mg IgG | ||
| GVA0-FZ6-010 | FragIT Z Mircospin
(Immobilized FabRICATOR® Z) |
2 x 0.5 mg | Immobilized FabRICATOR® Z enzyme on agarose beads |
| GVA0-FZ6-025 | 5 x 0.5 mg | ||
| GVA0-FZ6-050 | 10 x 0.5 mg | ||
| GVA2-FZ6-005 | FragIT Z kit | 0.5 mg
mouse IgG2a |
Rapid purification of F(ab’)2 and Fc/2 fragments from mouse IgG2a |
| GVA2-FZ6-025 | 5 x 0.5 mg
mouse IgG2a |
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| GVA0-FRC-050 | FabRICATOR®-HPLC | >200 injections | FabRICATOR-HPLC Column for automated subunit analysis |