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LumaScope Series

Lumascope series

인큐베이터 내에서 장시간 동안 세포 관찰이 가능한 고해상도 실시간 세포 이미지 시스템

 

 

 

 

 

 

 

 

 

 

 

 

ico_chk01  Bright field, Phase contrast, 3개 채널의 형광(Blue, Green, Red) 가능
ico_chk01 다양한 배율의 대물렌즈 선택 가능 (2.5x, 4x, 10x, 20x, 40x, 60x, 100x)
ico_chk01 세포 배양 인큐베이터, 조직 배양 후드 및 환경 워크스테이션 내부에서 사용 가능
ico_chk01 타임랩스 이미지, 실시간 비디오 제공
ico_chk01 5 Megapixel camera로 고해상도의 이미지 제공
ico_chk01 호환 가능한 용기 : Slide, Micro plate, T-flask, Dish

 

 

 

 

 

 

 

 

 

 

 

 

ico_chk01  Bright field, Phase contrast, 3개 채널의 형광(Blue, Green, Red) 가능
ico_chk01  실험 목적에 따라 다양한 배율의 대물렌즈 선택 가능 (2.5x, 4x, 10x, 20x, 40x, 60x, 100x)
ico_chk01  세포 배양 인큐베이터, 조직 배양 후드 및 환경 워크스테이션 내부에서 사용 가능
ico_chk01  Stitching & tiling을 위한 정밀도의 고속 스테이지
ico_chk01  Autofocus 및 Automated XY stage 가능
ico_chk01  타임랩스 이미지 및 Z-stack 이미지 가능
ico_chk01  자동 터렛 전환으로 최대 4개의 대물렌즈 장착 가능
ico_chk01  5 Megapixel camera로 고해상도의 이미지 제공
ico_chk01  호환 가능한 용기 : Slide, Micro plate(6 – 384well plate), T-flask, Dish

 

LS Microscopes (Lumascopes) can be used in practically any application where a standard inverted (and many upright) microscopes are used. Most of the applications below involve cells but LS Microscopes can also image beads and other materials.

 

ico_chk01  Live cell imaging
See Etaluma – Cardiac Myocytes Undergoing Division


ico_chk01  Cell growth and confluence

See Time Lapse Video of MSC in 2D Cell Culture


ico_chk01  Cell migration and wound healing

See Cell Migration & Wound Healing Application Note
See Migration of MSC in 2D Cell Culture


ico_chk01  Cell cycle protein expression

See Human HT1080 Fibrosarcoma Cells

ico_chk01  Use of micro-environmental systems
See Bioptechs products on Etaluma


ico_chk01  Determining transfection efficiency

See HUVEC Transfection Optimized with Cytofect™ Kit

 


In Vitro Exercise Model

Cultured skeletal muscle myotubes are electrically stimulated under hypoxic conditions and with temperature manipulations. Cell signal transduction dynamics are measured using proteomic techniques to help understand how exercise stressors are translated into fitness-promoting adaptions such as increased mitochondria. Probe in photo measures PO2 in the cell medium rather than in the atmosphere. LS620 allows visualization of contracting cells and assessment of their health.

Thank you to Dr David Clarke and his lab, Laboratory for Quantitive Exercise Biology, Simon Fraser University, British Columbia, Canada

LED Excitations 405 nm, 488 nm, 589 nm
Filter Set Blue : Excitation 370-410 nm, Emission 429-462 nm

Green : Excitation 473-491 nm, Emission 502-561 nm

Red : Excitation 580-598 nm, Emission 612-680 nm

Transmitted Modes Bright Field and Phase Contrast
Objectives 2.5x, 4x, 10x, 20x, 40x, 60x, 100x incl. oil immersion
Camera High Sensitivity Monochrome CMOS BSI Sensor; 5 megapixel 12-Bit
Image-Formats TIF, BMP, PNG, JPG
Image Size 100 x 100 up to 2100 x 2100 pixels (LS820 & LS850)

100 x 100 up to 1900 x 1900 pixels (LS620)

Video Rate 25 FPS (exposure limited)
Automated XY 120 mm x 80 mm travel, 1-3 micron positional reproducibility

(LS850 only)

Motorized Z 14 mm travel, 100 nm step, image-based autofocus, Z-stacks

(LS820 & LS850 only)

Throughput 96-well microplate, transmitted only, no AF :2.5 minutes

3 channel FI, no AF : 6.5 minutes