Bright field, Phase contrast, 3개 채널의 형광(Blue, Green, Red) 가능
실험 목적에 따라 다양한 배율의 대물렌즈 선택 가능 (2.5x, 4x, 10x, 20x, 40x, 60x, 100x)
세포 배양 인큐베이터, 조직 배양 후드 및 환경 워크스테이션 내부에서 사용 가능
Stitching & tiling을 위한 정밀도의 고속 스테이지
Autofocus 및 Automated XY stage 가능
타임랩스 이미지 및 Z-stack 이미지 가능
자동 터렛 전환으로 최대 4개의 대물렌즈 장착 가능
5 Megapixel camera로 고해상도의 이미지 제공
호환 가능한 용기 : Slide, Micro plate(6 – 384well plate), T-flask, Dish
LS Microscopes (Lumascopes) can be used in practically any application where a standard inverted (and many upright) microscopes are used. Most of the applications below involve cells but LS Microscopes can also image beads and other materials.
Live cell imaging
See Etaluma – Cardiac Myocytes Undergoing Division
Cell growth and confluence
See Time Lapse Video of MSC in 2D Cell Culture
Cell migration and wound healing
See Cell Migration & Wound Healing Application Note
See Migration of MSC in 2D Cell Culture
Cell cycle protein expression
See Human HT1080 Fibrosarcoma Cells
Use of micro-environmental systems
See Bioptechs products on Etaluma
Determining transfection efficiency
See HUVEC Transfection Optimized with Cytofect™ Kit
In Vitro Exercise Model
Cultured skeletal muscle myotubes are electrically stimulated under hypoxic conditions and with temperature manipulations. Cell signal transduction dynamics are measured using proteomic techniques to help understand how exercise stressors are translated into fitness-promoting adaptions such as increased mitochondria. Probe in photo measures PO2 in the cell medium rather than in the atmosphere. LS620 allows visualization of contracting cells and assessment of their health.
Thank you to Dr David Clarke and his lab, Laboratory for Quantitive Exercise Biology, Simon Fraser University, British Columbia, Canada
LED Excitations | 405 nm, 488 nm, 589 nm |
Filter Set | Blue : Excitation 370-410 nm, Emission 429-462 nm
Green : Excitation 473-491 nm, Emission 502-561 nm Red : Excitation 580-598 nm, Emission 612-680 nm |
Transmitted Modes | Bright Field and Phase Contrast |
Objectives | 2.5x, 4x, 10x, 20x, 40x, 60x, 100x incl. oil immersion |
Camera | High Sensitivity Monochrome CMOS BSI Sensor; 5 megapixel 12-Bit |
Image-Formats | TIF, BMP, PNG, JPG |
Image Size | 100 x 100 up to 2100 x 2100 pixels (LS820 & LS850)
100 x 100 up to 1900 x 1900 pixels (LS620) |
Video Rate | 25 FPS (exposure limited) |
Automated XY | 120 mm x 80 mm travel, 1-3 micron positional reproducibility
(LS850 only) |
Motorized Z | 14 mm travel, 100 nm step, image-based autofocus, Z-stacks
(LS820 & LS850 only) |
Throughput | 96-well microplate, transmitted only, no AF :2.5 minutes
3 channel FI, no AF : 6.5 minutes |